TY  - JOUR
T1  - Immunohistochemical Overexpression of nNOS, S100 Protein, and α-Synuclein in Myenteric Plexus of AS/AGU Rats
A1  - Muhammad Atteya
A1  - Aly M.Ahmed
A1  - Raeesa A. Mohammed
A1  - Musaad A. Alfayez
A1  - Shimaa A. Mohammed
A1  - Abdulaziz Siyal
A1  - Tahani A. Al-Matrafi
A1  - Amal AlRabiah
A1  - Hamad M. Alqahtani
A1  - Anthony Payne
JF  - International Journal of Pharmaceutical Research and Allied Sciences
JO  - Int J Pharm Res Allied Sci
SN  - 2277-3657
Y1  - 2020
VL  - 9
IS  - 3
SP  - 163
EP  - 171
N2  - Objective: There are four currently motor features characterizing Parkinson's disease (PD). These include rigidity of muscles, bradykinesia, tremors at rest, and instability of posture. Along the course of PD, the impairment of motor functions is commonly preceded by nonmotor symptoms (NMS) such as olfactory deficit, difficult swallowing (dysphagia), drooling (sialorrhea), constipation, urinary bladder dysfunction, depression, and sleep disorder. It was suggested that the enteric nervous system could be the initial site for the pathological process leading to PD. Materials and Methods: Six male adult control AS rats (normal control) and six male adults AS/AGU rats (model of PD) were sacrificed. A rectangular strip from the body of the stomach and a cross-section from the duodenum were dissected and processed for histological staining with hematoxylin and eosin, and immunohistochemical staining for detection of nNOS (neuronal NOS), S100 protein (astrocyte marker), and alpha-synuclein (α-synuclein). Results: The histological analysis of the stomach and duodenum of AS/AGU rats demonstrated necrotic smooth muscle cells of muscularis externa. The immunohistochemical analysis of AS/AGU rats showed a statistically significant increase in the expression of nNOS, S100 protein, and α-synuclein expression of myenteric plexuses compared to the control strain AS rats. Conclusion: Gastroduodenal tract of AS/AGU rats showed marked histopathological changes and immunohistochemical overexpression of nNOS, S100, and α-synuclein.
UR  - https://ijpras.com/article/immunohistochemical-overexpression-of-nnos-s100-protein-and-synuclein-in-myenteric-plexus-of-asagu-rats
ER  -