It has been well reported that fatigue is an important factor which hinders performance. The resultant effect of it is loss of co-ordination and efficiency. The main aim of this investigation was to find out the effect of fatigue on muscular co-ordination among rifle shooters. Ten rifle shooters aged between 22-28 years were selected as subjects. The index of muscular co-ordination was tested with three series. A steadiness tester was used with the diameters of 0.5, 0.31, 0.25, 0.18, 0.15, 0.12, 0.10, 0.09, 0.07 inches and stylus of 0.062. Grip dynamometer was utilized for measuring local fatigue. The results indicated that 50% of rifle shooters were adversely affected by local fatigue of the arm, 80% of the subjects stated alteration in their muscular co-ordination after overall fatigue was induced. It was concluded that performance was substantially reduced when general fatigue was induced compared to local fatigue.