Emotional intelligence exerts a noteworthy impact on both turnover intentions and job satisfaction. The objective of this research was to evaluate the perceived emotional intelligence and its relationship with work life balance and job satisfaction among the healthcare professionals in twin cities of Pakistan. A descriptive cross sectional study design was used. Two different pre validated tools, the impact of work life balance factors on job satisfaction questionnaire, and the NHS emotional intelligence questionnaire were distributed to conveniently selected sample of 283 prescribers, 214 nurses, and 215 pharmacists. After the data collection, the data was cleaned, coded and entered in SPSS version 21, and was statistically analyzed. Job satisfaction had a statistically significant linear weak negative correlation (p< .001) with self awareness, emotion management, empathy, social skills and motivation. On the other hand, work life balance had a statistically significant “very weak negative correlation” (p< .001) with emotion management, empathy and motivation. However, a “moderate positive correlation” (p< .001) existed between job satisfaction and work life balance. The results of the present study concluded that a significant relationship existed among the emotional intelligence, work life balance and job satisfaction. The emotionally intelligent healthcare professionals were better in managing their work life balance and were more satisfied with their job. Pharmacists possessed better social skills with higher motivation levels while physicians were better in managing work life balance than all the other healthcare professionals in Pakistan.