Phenolics Compounds and Biological Activity of Leaves of Anabasis Articulata, ‎an Algerian Medicinal Plant

Elhouaria‏ ‏Benzineb , Nadia Kambouche , Abdelkader Hamiani , Salima‏ ‏Bellahouel , ‎Hanane Zitouni , Houari‏ ‏Toumi

Abstract

Anabasis articulata is a Saharan plant used by Algerian traditional medicine practitioners for medicinal purposes. The objective of this study was to investigate the antioxidant properties of the extract and fractions of the leaves of A. articulata. The phenolic compounds were also quantified in this plant. Anabasis articulata was examined for antibacterial activities of its methanolic extract using disc diffusion method. The bacteria tested include Pseudomonas aeruginosa ATCC 14028, Salmonella typhimurium ATCC 9027 and Bacillus subtilis spizizenii ATCC 6633. The results showed that methanolic extract exhibits a higher level of phenolic compounds (230.000±0,415) as compared to all other extracts. The FRAP test revealed that the methanolic extract has a higher reducing power (0.1 mg / ml) compared to other extracts. IC50 (DPPH) was ranged from 3.200±0.088 to 4.900 ± 0.130. The compounds quantified by HPLC in the crude extract and fractions were quercetin and ascorbic acid. The results obtained indicated that A. articulata exhibits a good potential to prevent diseases and it might also be used as a natural preservative for food or cosmetic products.