Synthesis and Biological Evaluation of Some Novel Hybrid Quinazolinone–Piperazine Derivatives as Anti-Microbial Agents

The chemical constituents quinazolinone and piperazine hold significant importance in the realm of organic compounds due to their diverse range of biological and therapeutic attributes. In an effort to investigate the possible uses of these molecules, a team of scientists synthesized a unique set of chemical substances that combined piperazine and quinazolinone structures. This research involved a comprehensive investigation into the antimicrobial properties of a set of derivatives bearing the chemical structure N-(4-oxo-2-(4-(4-(2-(Substituted phenylamino) acetyl) piperazin-1-yl) phenyl) quinazolin-3(4H)-yl) benzamide, which was successfully synthesized with high yields. The synthesized compounds were carefully characterized using a range of physical methods, including mass spectrometry, FTIR, and ¹H NMR spectroscopy, in addition to physical methods such as melting point measurement and thin-layer chromatography. Subsequently, using the agar well diffusion method, these compounds were evaluated for their antibacterial activity against a panel of microbial strains, including Staphylococcus aureus, Bacillus subtilis, Escherichia coli, and Pseudomonas aeruginosa. The antifungal assay was also conducted against Candida albicans using the same approach. The broth microdilution technique was also used to establish the minimal inhibitory concentration. The synthesized compounds demonstrated impressive antifungal and antibacterial properties against every tested microbe. Notably, among the compounds evaluated, PRP7A6, PRP7A8, and PRP7A11 displayed the most potent antimicrobial effects against the pathogenic strains.