Ghena Mohammad Abdrabou Mohammad Al-Jahani

Abstract

Thymus Vulgaris (Thyme) is a natural organic matter to biosynthesis silver nanoparticles (AgNPs) and their antibacterial efficiency. This research deals with the green synthesis of AgNPs by Thyme extract. Nanoparticles were characterized using UV-Vis spectra to analyze size and shape regulated nanoparticles in aqueous suspension, AAS to observe the varying concentricity of Ag+ ions in the solution through time, and FTIR to classify the possible Les combination’s current activities in Thyme extract that are responsible for the silver reduction. The activity of antibacterial nanoparticles against pathogenic gram-positive and negative bacteria was studied. Additionally, an edible film of methyl cellulose reinforced with AgNPs to evaluate its antibacterial efficiency to utilized as alimentary packaging substances U.V. AgNPs reduced thyme extract and became globular intense peaks centered near 410 nm, thyme extract reduced the silver ions to silver nanoparticles decrease in the concentration of Ag+ ions from 5.5 ppm to 0.05 ppm for 12 minutes the reaction to occur. FTIR, the presence of carbonyl group of amino acids of proteins and -HO stretching in phenol extract of thyme leaves acts as a reducing agent and stabilizing silver nanoparticles and protecting them from changes, the antibacterial activity of biosynthetic AgNPs from thyme shows a promising area of inhibition on pathogenic gram-positive and negative bacteria. Also, as observed clear bactericidal activity of the cellulose / AgNPs mixture membranes against Escherichia coli 0157: H7 after 12 h of incubation at 37 o-C and increased with increasing concentration of AgNPs.