UV-Spectropectrophotometry for Determination of Diazepam by Comparative Estimation of Methods of Calibration Curve and Reference Standard

The objective of the current work was to compare the methods of the calibration curve (MCC) and the external reference standard (MRS) for estimating the analytical parameters accuracy and precision in order to validate the UV-spectrophotometric method for the determination of diazepam at λmax = 328 nm in 95% ethanol. The fact that the detected absorbance at λmax = 328 nm, a particular wavelength for diazepam, could not be found in the UV spectra of the blank solution was provided as evidence of selectivity. Analysis using linear regression was performed on the experimental findings: y = 14387.x +0.05. An analysis of linearity was conducted by measuring the linear regression coefficient: R² > 0.998. LOD = 1.84.10^–5 g/ml; LOQ = 1.04.10^–5 g/ml. Accuracy was reflected and determined by recovery degree R [%] ± RSD [%] as per ICH guidelines: MCC: 100.49 % ± 1.35 %; MRS: 105.56 % ± 1.37 %. The fact that SD and RSD were less than 1.5 confirmed that the findings and actual values were in agreement. From the precision assessment, all values for the content of Diazepam, obtained both by MRS and MCC at the confidence possibility P = 98%, suit the relevant confidence interval: MCC: 4.88 mg ¸ 4.96 mg; MRS: 5.12 mg ¸ 5.20 mg. Through the use of all of these data, it was demonstrated that the validated approach was reliable and accurate for determining the dose of Diazepam in medication formulations.