Abstract

The present study was aimed to evaluate the antidiabetic potential of combination of punica granatum peel extract, spilanthes paniculata flower extract and selenium in streptozotocin (STZ) induced diabetes in mice. Group A was used as control and Group B was used as diabetic control (STZ, 40mg/kg for 3 days, i.p.). A placebo was given to both the group orally. Metformin (120mg/kg) was given intraperitoneally as standard drug to Group C. Ethanolic peel extract of punica granatum (EPP 600mg/kg, p.o.) was administered to group D, (EPP) in combination with ethyl acetate extract of spilanthes paniculata flower (EAS) was administered to group E (EPP 300mg/kg, p.o. + EAS 200mg/kg, p.o.), Sodium selenite was given with combination of herbal extract (EPP 300 mg/kg, p.o.+ EAS 200mg/kg, p.o. + Se 0.3mg/kg, i.p.) to group F. STZ was given in multiple low doses of 40mg/kg, i.p. for 3 consecutive days & hyperglycemia was observed on 7th day. Blood glucose was determined on 0th, 7th, 14th, and 21st day. Diabetic mice in all group showed significant (P<0.01) increase in serum blood glucose as compared to control animals. Blood glucose level of animals treated with metformin & herbo-mineral combination lowers the glucose level significantly (P<0.01) as compared to diabetic control. The herbo-mineral combination shown significant (P<0.01) increase in the antioxidant enzymes Catalase, SOD, GSH and reduction in MDA & NO levels as compared to diabetic control. Thus, the present study indicates decreased blood glucose level & significant improvement in the biomarkers of oxidative stress by using the combination of sodium selenite with herbal extracts. The results confirm that the herbo-mineral combination is not only useful in controlling blood glucose level but also helps to prevent oxidative stress induced damage.