Isolation and extraction of curcumin from three different varieties of curcuma longa L - a comparative study


Curcuminoids, is one of the naturally occurring yellow pigments which consist of curcumin, demethoxycurcumin and bisdemethoxycurcumin, in Curcuma longa rhizome have been popularly used in traditional medicine and some extent, it is one of the active indigredients in drugs and cosmetics. In this study, natural dye curcumin was extracted from 3 differenent varieties of 10 different rhizomes. Among 3 varieties, salem variety large finger (1.92 %) and bulb(1.88%) shows higher yield of curcurmin. Where as No. 8 variety, in large finger (1.81%) and bulb (1.46%) of curcumin. In case of Erode variety, 1.36% from large finger and 1.33% from bulb. For separation of curcumin from demethoxycurcumin and bisdemethoxycurcumin, the mobile phase was prepared using chloroform and methanol in the ratio of 95:5 respectively. The maximum absorbance of the standard curcuminoid was observed at the wavelength of 420 nm with the absorbance of 0.545. Similarly, UV absorption spectra of natural dye solution exteracted from Curcuma longa.L was 0.743 at 420 nm. From the study, data are useful for further development of standard procedures. Whis this, we can improve extraction efficiency and purification of curcurmin from Curcuma longa. L.