One of the methods of cancer treatment is radiotherapy. Although radiation resistance and toxicity in normal cells limit the use of radiotherapy in some specific anatomical situations, the use of substances that increase the radiation sensitivity of cancer cells while minimizing toxicity to normal cells can be beneficial. The present study aimed to investigate the radio-sensitizing effect of tolmetin in radiotherapy treatment on human colon cancer cells. In this study, human clone HT-29 cancer cells in different groups were divided into different groups and exposed to X-rays and tolmetin drugs. They were then compared with each other and with the control group using the micronucleus evaluation method and the nuclear division index. The effect of cytotoxicity was evaluated using the nuclear division index and the genotoxicity of cells was assessed by evaluating the number of micronuclei. Based on the results, the number of micronuclei increased significantly in the radiation-receiving group compared to the control group. In the group receiving tolmetin at concentrations of 75 and 100 μM, the number of micronuclei also increased compared to the control group. A significant increase in the number of micronuclei was observed in all groups treated with tolmetin that received radiation, with the increase being most pronounced at concentrations of 100 and 150 μM. Meanwhile, tolmetin did not change the nuclear division index in the studied concentrations. The present study showed that tolmetin has a radiosensitizing effect on HT-29 human colon cancer cells, and this effect is concentration-dependent. In addition, tolmetin was found not to exhibit cytotoxicity in the mentioned cell line.
IJPRAS
