Antioxidant, Thrombolytic, and Neuropharmacological Studies on Methanol Extract of Macaranga Indica Wight Leaves
Abstract
The current study aimed to assess the phytochemical profile of Macaranga indica (MI) leaves, as well as their antioxidant, thrombolytic, and neuropharmacological activities. Methanol extract from Macaranga indica (MEMI) Wight leaves was used to investigate these activities to validate the therapeutic profile using a well-established method. GraphPad Prism, version 9.4.1 was used to analyze the experimental results. In vitro, the antioxidant DPPH free radical scavenging assay yielded an IC50 value of 9.58 µg/ml. Reducing power capacity showed an increase in absorbance with the increase of different concentrations. Quantitative analyses of phytochemical elicit the presence of phenol (777±12.13), flavonoid (99.94±0.62), flavonol (244±2.53), total antioxidant (184.48±1.92) mg/g in the dry extract. In vitro thrombolytic activity showed a highly statistically significant (P < 0.0001) clot lysis (42.66±3.19%). In vivo antidepressant, anxiolytic, and sedative activities evaluated the statistically significant (P < 0.001 at 200 mg/kg and P < 0.0001 at 400 mg/kg) by decreasing immobility time of forced swimming test, (P < 0.0001) by reducing anxiety of elevated plus maze and hole board test and (P = 0.0106) mice movement were gradually decreasing of hole cross test. These findings indicate that the crude extract of MEMI leaves has significant antioxidant, thrombolytic, and neuropharmacological properties.
Keywords: Macaranga indica wight, Methanol, Antioxidant, Thrombolytic, Neuroprotective
