2024 Volume 13 Issue 1
Creative Commons License

Mining of Gene Modules and Identification of Key Genes for Early Diagnosis of Gastric Cancer


, , , , , , , ,
  1. College of Life Sciences, Xinyang Normal University, Xinyang 464000, China.
  2. School of Medicine, Chongqing University, Chongqing 400044, China.
  3. Chongqing Key Laboratory of Translational Research for Cancer Metastasis and Individualized Treatment, Chongqing University Cancer Hospital, Chongqing 400030, China.
  4. Department of Computer Science, City University of Hong Kong, Hong Kong, China.
Abstract

Gastric cancer (GC) is one of the most common malignant tumors with high incidence and mortality rates. Most patients with GC are not diagnosed until the advanced stage of cancer or during tumor screening, resulting in missing the best treatment time. This study identified key modules and hub genes associated with GC by weighted gene co-expression network analysis (WGCNA). The "limma" package in R was used to identify differentially expressed genes (DEGs) in GC samples from TCGA, and a total of 4892 DEGs were identified. GO enrichment and KEGG pathway enrichment analyses were conducted to detect the related pathways and functions of DEGs. These DEGs were primarily associated with extracellular matrix organization, DNA replication, cell cycle, and p53 signaling pathway. Gene modules associated with clinical characteristics were identified with WGCNA in tumor and normal samples. Six gene modules were obtained in the WGCNA network, of which two modules were significantly correlated with GC. Hub genes of key modules were identified using survival analysis and expression analysis. Finally, one-way ANOVA was used to explore the relationship between hub gene expression in normal tissues and different pathological stages of GC. Through survival and expression analysis, a total of 19 genes with good prognosis and significantly differential expressed were identified. The hub genes were significantly differential expressed in normal tissues and different pathological stages of GC, indicating that these genes have important diagnostic value for early GC and can be used as auxiliary indicators in the diagnosis of early GC.


How to cite this article
Vancouver
Xu L, Yang J, Zhang Y, Liu X, Liu Z, Sun F, et al. Mining of Gene Modules and Identification of Key Genes for Early Diagnosis of Gastric Cancer. Int J Pharm Res Allied Sci. 2024;13(1):26-38. https://doi.org/10.51847/MFOQLj1g2f
APA
Xu, L., Yang, J., Zhang, Y., Liu, X., Liu, Z., Sun, F., Ma, Y., Wang, L., & Xing, F. (2024). Mining of Gene Modules and Identification of Key Genes for Early Diagnosis of Gastric Cancer. International Journal of Pharmaceutical Research and Allied Sciences, 13(1), 26-38. https://doi.org/10.51847/MFOQLj1g2f
Related articles:
Most viewed articles:
Issue 3 Volume 13 (2024)